Abstract
The complete genome sequence of maize mosaic virus (MMV) was obtained using next-generation sequencing from infected Peregrinus maidis and rapid amplification of cDNA ends from infected Zea mays The genome of MMV is 12,170 bases, and this project completed the 5' and 3' ends and amended the polymerase sequence.
Highlights
The complete genome sequence of maize mosaic virus (MMV) was obtained using next-generation sequencing from infected Peregrinus maidis and rapid amplification of cDNA ends from infected Zea mays
Martin and Whitfield full leader sequences, 3= rapid amplification of cDNA ends (RACE) of the viral genome was done by adding poly(A) sequences to the leader utilizing Escherichia coli poly(A) polymerase (New England BioLabs, Ipswich, MA) and SuperScript III (Invitrogen, Carlsbad, CA) for first-strand cDNA synthesis and amplification with a poly(T) primer and an N-specific primer (5=-GCAGT CGCCAAATTAGTCCAGTC-3=)
The data for this paper are available at the NCBI as the completed genome under GenBank accession number MK828539, and the MMV-infected P. maidis RNA sequencing (RNA-Seq) library sequences are under SRA project number PRJNA540525
Summary
The complete genome sequence of maize mosaic virus (MMV) was obtained using next-generation sequencing from infected Peregrinus maidis and rapid amplification of cDNA ends from infected Zea mays. Received 28 May 2019 Accepted 28 June 2019 Published 18 July 2019 mra.asm.org 1 Martin and Whitfield full leader sequences, 3= RACE of the viral genome was done by adding poly(A) sequences to the leader utilizing Escherichia coli poly(A) polymerase (New England BioLabs, Ipswich, MA) and SuperScript III (Invitrogen, Carlsbad, CA) for first-strand cDNA synthesis and amplification with a poly(T) primer and an N-specific primer (5=-GCAGT CGCCAAATTAGTCCAGTC-3=).
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