Abstract
The complete genome sequence of Lactococcus lactis subsp. cremoris 3107, a dairy starter strain and a host for the model lactococcal P335 bacteriophage TP901-1, is reported here. The circular chromosome of L. lactis subsp. cremoris 3107 is among the smallest genomes of currently sequenced lactococcal strains. L. lactis subsp. cremoris 3107 harbors a complement of six plasmids, which appears to be a reflection of its adaptation to the nutrient-rich dairy environment.
Highlights
The complete genome sequence of Lactococcus lactis subsp. cremoris 3107, a dairy starter strain and a host for the model lactococcal P335 bacteriophage TP901-1, is reported here
Library samples were loaded into a flow cell version 3 (600 cycles; Illumina), and draft genome sequencing was performed on a MiSeq genomic platform (Illumina, UK) at GenProbio srl (Parma, Italy)
Fastq files of the paired-end reads obtained from the genome sequencing were used as input for genome assemblies through the MEGAnnotator pipeline [8]
Summary
The complete genome sequence of Lactococcus lactis subsp. cremoris 3107, a dairy starter strain and a host for the model lactococcal P335 bacteriophage TP901-1, is reported here. For Illumina-based sequencing, 5 g chromosomal DNA from L. lactis subsp. Genomic libraries were constructed using the TruSeq DNA PCR-free LT kit (Illumina) and 2.5 g of genomic DNA, which was fragmented with a Bioruptor next-generation sequencing (NGS) ultrasonicator (Diagenode, USA), followed by size evaluation using the TapeStation 2200 system (Agilent Technologies). Fastq files of the paired-end reads obtained from the genome sequencing were used as input for genome assemblies through the MEGAnnotator pipeline [8].
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