Complete Genome Sequence of Lactobacillus crispatus AB70, Isolated from a Vaginal Swab from a Healthy Pregnant Korean Woman.

Dong-Ho Chang,In-Hwa Chung,Byoung-Chan Kim,Haeyoung Jeong,Moon-Soo Rhee,Sung-Ki Lee,David Rasko

doi:10.1128/mra.01736-18

Abstract

The vaginal bacterial strain AB70, belonging to the species Lactobacillus crispatus, was isolated from a vaginal swab from a healthy pregnant Korean woman. Here, we report the 2.37-Mb complete genome sequence of this strain.

Highlights

The vaginal bacterial strain AB70, belonging to the species Lactobacillus crispatus, was isolated from a vaginal swab from a healthy pregnant Korean woman
The genome most similar to AB70 among all 57 publicly available L. crispatus genomes in the RefSeq database, identified with dRep [12] version 2.0.5, was that of strain UMB0803 (GenBank number GCF_002861765, 99.7% average nucleotide identity [ANI]), one of the nine L. crispatus strains isolated from female urine collected with a transurethral catheter (BioProject number PRJNA316969, 200 strains)
We identified two bacteriocin biosynthetic gene clusters with antiSMASH version 4.1.0 [14], one of which was similar to a gassericin T [15] biosynthetic gene cluster

Summary

Introduction

The vaginal bacterial strain AB70, belonging to the species Lactobacillus crispatus, was isolated from a vaginal swab from a healthy pregnant Korean woman. One of the isolates, named strain AB70, displayed strong antibacterial activities and was selected for further investigation, including complete genome analysis. The genomic DNA of strain AB70 was extracted from 1-liter anaerobic MRS liquid cultures, as previously described [5].

Results

Conclusion