Abstract
Host-associated treponeme bacteria play etiological roles in human and animal soft tissue infections. Treponema sp. strain OMZ 804 (ATCC 700766) was isolated from dental plaque sampled from a patient with periodontitis in Switzerland in 1994. We report here the complete genome sequence of its 2.98-Mb circular chromosome.
Highlights
The host-associated treponeme bacteria Treponema medium and Treponema vincentii play etiological roles in oral infectious diseases in humans and animals, especially periodontal diseases, and in various animal soft tissue infections [1,2,3,4,5]
Long-read sequencing was performed on an Oxford Nanopore Technologies (ONT) minION Mk1B device with an R9.4 flow cell (FLO-MIN106)
The DNA was repaired using the NEBNext formalin-fixed, paraffinembedded (FFPE) repair mix (New England Biolabs), cleaned with AMPure XP beads (Beckman Coulter), and dA tailed using the NEBNext end repair/dA-tailing module; sequencing adapters were ligated onto the prepared ends
Summary
The host-associated treponeme bacteria Treponema medium and Treponema vincentii play etiological roles in oral infectious diseases in humans and animals, especially periodontal diseases, and in various animal soft tissue infections [1,2,3,4,5]. Long-read sequencing was performed on an Oxford Nanopore Technologies (ONT) minION Mk1B device with an R9.4 flow cell (FLO-MIN106). The whole-genome sequencing library was prepared using the ONT 1D genomic DNA ligation sequencing kit (SQK-LSK108) and barcoding kit (EXP-NBD103) according to the manufacturer’s protocol (version NBE_9006_v103_revP_21Dec2016), excluding the optional genomic DNA fragmentation step. ONT reads were base called with Albacore v2.3.3 [11], and adapters were removed from the sequence reads using Porechop v0.2.3 [12].
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