Abstract
The industrially important thermophile Geobacillus thermoglucosidasius has the potential to produce chemicals and fuels from biomass-derived sugar feedstocks. Here, we present the genome sequence of strain NCIMB 11955, the progenitor of an ethanologenic industrial strain, revealing 11 single-nucleotide polymorphisms and 2 indels compared to strain DSM 2542 and two novel plasmids.
Highlights
The industrially important thermophile Geobacillus thermoglucosidasius has the potential to produce chemicals and fuels from biomass-derived sugar feedstocks
As phenotypes vary widely within the same strain due to genotypic changes [9, 10], we have sequenced the direct progenitor of TM242, NCIMB 11955
Paired-end libraries were sequenced using an Illumina MiSeq bench-top sequencer (Deepseq, University of Nottingham, Nottingham, UK), producing 1.66 Gb of data consisting of a total of 801,181,830 nucleotides (3,203,788 reads with an average length of 250.1 bp)
Summary
The industrially important thermophile Geobacillus thermoglucosidasius has the potential to produce chemicals and fuels from biomass-derived sugar feedstocks. The genome sequence of strain DSM2542, originally isolated in Japan [6, 7], has already been published [8]. Paired-end libraries were sequenced using an Illumina MiSeq bench-top sequencer (Deepseq, University of Nottingham, Nottingham, UK), producing 1.66 Gb of data consisting of a total of 801,181,830 nucleotides (3,203,788 reads with an average length of 250.1 bp).
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