Abstract
Staphylococcus aureus multilocus sequence type 398 (ST398) is responsible for an increasing number of severe infections in humans. There are no reports detailing if all ST398 strains are equally virulent. We present the genome sequence of the moderate-virulence ST398 methicillin-susceptible Staphylococcus aureus strain GD1108, determined in a Caenorhabditis elegans infection model, to reveal the ST398 sublineage virulence.
Highlights
Staphylococcus aureus multilocus sequence type 398 (ST398) was first reported as an animal pathogen; it gained notoriety when it was found associated with human diseases, ranging from minor localized infections to more severe invasive illnesses (1–17)
Filtered PacBio subreads and trimmed Illumina reads were used for hybrid genome assembly using the Unicycler v0.4.7 pipeline (SPAdes v3.13.0, minimap, Racon v1.3.2, Pilon v.1.23) (20–24)
GC content was determined with QUAST v4.4, and gene annotation was accomplished using NCBI’s Prokaryotic Genome Annotation Pipeline using the bestplaced reference protein set (GeneMarkS-2ϩ v4.8) (25, 26)
Summary
Staphylococcus aureus multilocus sequence type 398 (ST398) was first reported as an animal pathogen; it gained notoriety when it was found associated with human diseases, ranging from minor localized infections to more severe invasive illnesses (1–17). Filtered PacBio subreads (prepared by Genome Quebec) and trimmed Illumina reads were used for hybrid genome assembly using the Unicycler v0.4.7 pipeline (SPAdes v3.13.0, minimap, Racon v1.3.2, Pilon v.1.23) (20–24).
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
