Abstract

Podophage Pisces was isolated against Escherichia coli strain 4s from wastewater samples. Pisces is a T7-like phage, and all 49 predicted protein-coding genes in it are present on a single strand and are surrounded by 190-bp terminal repeats. Due to its similarity to other T7-like phages, 61% of Pisces genes were assigned a predicted function.

Highlights

  • Podophage Pisces was isolated against Escherichia coli strain 4s from wastewater samples

  • IMM-002 and its relatives are all T7-like phages

  • The Pisces genome was reopened at short direct terminal repeats of 190 bp predicted by PhageTerm [19], as expected for a T7-like phage

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Summary

Introduction

Podophage Pisces was isolated against Escherichia coli strain 4s from wastewater samples. Pisces has a 39,497-bp genome of 53.2% GϩC content with 49 predicted proteincoding genes on a single strand at 92.1% coding density. The closest relative to Pisces with 83.88% nucleotide identity is Escherichia phage IMM-002

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Conclusion
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