Abstract

ABSTRACTBacteriophage PGT2 was isolated from horse feces by using an uncharacterized Escherichia coli strain, 7s, isolated from the same sample as the host. Bacteriophage PGT2 and a related phage, phiKT, which was previously isolated from the same source, are likely to represent a new genus within the Autographivirinae subfamily of the Podoviridae family of viruses.

Highlights

  • Bacteriophage PGT2 was isolated from horse feces by using an uncharacterized Escherichia coli strain, 7s, isolated from the same sample as the host

  • The receptor recognition domains of both phiKT and PGT2 are related to recently characterized domains of lateral tail fibers of T5-related phages that were isolated from the same site [6], highlighting the importance of the characterization of ecologically linked phage isolates

  • Since the bacteriophages related to T7 have conserved sequences on the genome termini and possess 100- to 300-bp terminal repeats (TR), we set the ends of PGT2 at the same conserved sequences as those of phiKT

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Summary

Introduction

Bacteriophage PGT2 was isolated from horse feces by using an uncharacterized Escherichia coli strain, 7s, isolated from the same sample as the host. Bacteriophage PGT2 was grown and its DNA was extracted as described previously [3]. The DNA was sequenced using a Roche 454 Junior sequencer, yielding 9,893 reads of an average length of 417 bp.

Results
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