Abstract
Here, we describe the complete genome sequence of siphophage LL5. LL5 is a T1-like phage isolated against enterotoxigenic Escherichia coli, which causes traveler's diarrhea. LL5 is included as a component phage in the commercial prebiotic product PreforPro.
Highlights
Presence of Enterotoxigenic Escherichia coli (ETEC) strains can induce traveler’s diarrhea (TD), resulting in watery diarrhea [1] which may be accompanied by nausea, vomiting, abdominal pain, fever, or blood in stool [2]
Phage LL5 was isolated by the enrichment method [4] from filter-sterilized (0.22-m) influent from a municipal water treatment facility in College Station, TX, against a clinical ETEC isolate
Phage LL5 DNA was prepared using the GS FLX Titanium general DNA library preparation kit and was sequenced by FLX Titanium 454 pyrosequencing at the Emory-Georgia Research Alliance Genomics Center (Emory University, GA); trimmed sequence reads were assembled into a single contig at 19.9-fold coverage using Newbler version
Summary
Presence of ETEC strains can induce traveler’s diarrhea (TD), resulting in watery diarrhea [1] which may be accompanied by nausea, vomiting, abdominal pain, fever, or blood in stool [2]. Phage LL5 was isolated by the enrichment method [4] from filter-sterilized (0.22-m) influent from a municipal water treatment facility in College Station, TX, against a clinical ETEC isolate. The phage DNA was purified from high-titer lysates by a modified Wizard DNA purification kit (Promega) as previously described [5]. Phage LL5 DNA was prepared using the GS FLX Titanium general DNA library preparation kit and was sequenced by FLX Titanium 454 pyrosequencing at the Emory-Georgia Research Alliance Genomics Center (Emory University, GA); trimmed sequence reads were assembled into a single contig at 19.9-fold coverage using Newbler version
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