Abstract
Enterobacter sp. IIT-BT 08 belongs to Phylum: Proteobacteria, Class: Gammaproteobacteria, Order: Enterobacteriales, Family: Enterobacteriaceae. The organism was isolated from the leaves of a local plant near the Kharagpur railway station, Kharagpur, West Bengal, India. It has been extensively studied for fermentative hydrogen production because of its high hydrogen yield. For further enhancement of hydrogen production by strain development, complete genome sequence analysis was carried out. Sequence analysis revealed that the genome was linear, 4.67 Mbp long and had a GC content of 56.01%. The genome properties encode 4,393 protein-coding and 179 RNA genes. Additionally, a putative pathway of hydrogen production was suggested based on the presence of formate hydrogen lyase complex and other related genes identified in the genome. Thus, in the present study we describe the specific properties of the organism and the generation, annotation and analysis of its genome sequence as well as discuss the putative pathway of hydrogen production by this organism.
Highlights
Hydrogen has great promise in contributing substatially to the renewable energy demands of the future
In this report we present a summary of the properties and features of Enterobacter sp
A set of standard tests were carried out according to Bergey’s Manual and the results showed that the strain belongs to Enterobacter species. 16S rRNA sequencing by Microbial Type Culture Collection (MTCC), Chandigarh further confirmed the strain identity
Summary
Hydrogen has great promise in contributing substatially to the renewable energy demands of the future. 1999 22 02’ 30” 87 11’ 0” NA NA a) MIGS: The minimum information about a genome sequence b) Evidence codes - IDA: Inferred from Direct Assay; TAS: Traceable Author Statement (i.e., a direct report exists in the literature); NAS: Non-traceable Author Statement (i.e., not directly observed for the living, isolated sample, but based on a generally accepted property for the species, or anecdotal evidence). These evidence codes are from the Gene Ontology project [21]. B) Pseudogenes may be counted as protein coding or RNA genes, so is not additive under total gene count
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