Abstract

ABSTRACTCupriavidus species have been known as versatile microorganisms in the field of industrial biotechnology. Cupriavidus sp. strain EM10 was isolated from sewage sludges. Here, we report the complete genome sequence of this bacterium, which contains 6,658,510 bp (GC content, 65.12%) and 6,248 genes.

Highlights

  • M embers of the genus Cupriavidus, belonging to the family Burkholderiaceae, have received much attention as bioplastic producers [1, 2] and potential bioagents for the bioremediation of heavy metal- and xenotoxic compound-contaminated environments [3]

  • The prepared library was sequenced on the PacBio RS II platform using PacBio P6-C4 chemistry in 8-well single-molecule real-time (SMRT) v3 cells [4]

  • Default parameters were used for all software unless otherwise specified

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Summary

Introduction

M embers of the genus Cupriavidus, belonging to the family Burkholderiaceae, have received much attention as bioplastic producers [1, 2] and potential bioagents for the bioremediation of heavy metal- and xenotoxic compound-contaminated environments [3]. Genome sequencing was performed by Chunlab, Inc. The DNA library preparation was performed using the PacBio DNA template prep kit 1.0 (Pacific Biosciences, USA). 10 mg genomic DNA was sheared and size-selected to 20 kb using g-TUBE devices (Covaris, Inc., Woburn, MA, USA).

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