Complete Genome Sequence of Community-Associated Methicillin-Resistant Staphylococcus aureus Sequence Type 1, SCCmec IV[2B], Isolated in the 1990s from Northern Western Australia.

Nicola M Karakatsanis,Julie C Pearson,Joshua P Ramsay,Elena Colombi,Geoffrey W Coombs,Shakeel Mowlaboccus

doi:10.1128/mra.00796-21

Abstract

ABSTRACTSequence type 1 (ST1) methicillin-resistant Staphylococcus aureus (MRSA) SCCmec IV[2B] has become one of the most common community-associated MRSA clones in Australia. We report the complete genome sequence of one of the earliest isolated Australian S. aureus ST1-MRSA-IV strains, WBG8287, isolated from an Indigenous Australian patient living in the remote Kimberley region of Western Australia.

Highlights

Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) first emerged in Australia in the 1980s [1, 2]
The assembly was polished with the MinION reads 6Â using Minimap2 v2.17-r941 [5] and Racon v1.4.15
The WBG8287 sequences are deposited in NCBI GenBank under accession numbers CP070986.1, CP070987.1, and CP070988.1

Summary

Introduction

Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) first emerged in Australia in the 1980s [1, 2]. The WBG8287 genome was sequenced using the ONT SQK-RAD004 kit, the ONT MinION R.9.4.1 FLO-MIN106 flow cell, and the MinION Mk1B (ONT, UK), using MinKNOW v20.10.3 and MinKNOW Core v4.1.2 software.

Results

Conclusion