Abstract
ABSTRACTHere, I report the complete genome sequence of Campylobacter jejuni strain G1, belonging to Penner serotype HS1. One remarkable feature of the genome of this isolate is the presence of four copies of Mu-like prophages, of which none are present in some other strains, including the reference strain NCTC11168.
Highlights
Campylobacter jejuni strain G1, isolated from a Guillain-Barré syndrome patient [1], has the smallest known capsular polysaccharide locus [2,3,4,5,6]
Of 2 Â 250,566 paired short reads produced by the Illumina NovaSeq 6000 platform using SPAdes software v.3.7 resulted in 25 contigs (1 to 406 kb); the N50 value was 120,957 bp
Long read genomic DNA libraries were prepared with the Oxford Nanopore Technologies (ONT; United Kingdom) SQK-RBK004 kit and/or SQK-LSK109 kit with the native barcoding EXP-NBD104/ 114 (ONT) kit with 500 ng of DNA
Summary
Campylobacter jejuni strain G1, isolated from a Guillain-Barré syndrome patient [1], has the smallest known capsular polysaccharide locus [2,3,4,5,6]. A sequencing DNA library was prepared using the Nextera XT library prep kit (Illumina, San Diego, CA) following the manufacturer’s protocol. Of 2 Â 250,566 paired short reads (average, 228 bp) produced by the Illumina NovaSeq 6000 platform using SPAdes software v.3.7 resulted in 25 contigs (1 to 406 kb); the N50 value was 120,957 bp.
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