Abstract
Here, we describe the complete genome sequence of Caldicellulosiruptor changbaiensis, isolated from a hot spring in the Changbai Mountain Range of China. Currently, only one other genome sequence representing a Caldicellulosiruptor species from China is available. Assembly of a continuous single contig used both Oxford Nanopore and Illumina sequencing data.
Highlights
We describe the complete genome sequence of Caldicellulosiruptor changbaiensis, isolated from a hot spring in the Changbai Mountain Range of China
Long-read sequencing libraries were constructed with a rapid sequencing kit (SQKRAD004, Oxford Nanopore Technologies) and 400 ng of genomic DNA (gDNA) per the manufacturer’s instructions
Adapters were trimmed with PoreChop v0.2.4, and sequence data were further analyzed and subsampled with NanoPlot v1.15.0 and NanoFilt v2.2.0 [15] based on a quality score of 11 and a read size greater than 4,000 bp, which resulted in 171 Mbp of data for assembly
Summary
We describe the complete genome sequence of Caldicellulosiruptor changbaiensis, isolated from a hot spring in the Changbai Mountain Range of China. Long-read sequencing libraries were constructed with a rapid sequencing kit (SQKRAD004, Oxford Nanopore Technologies) and 400 ng of gDNA per the manufacturer’s instructions. After loading the library into a R9.4.1 flow cell for the MinION platform (FLO-MIN106), sequence data collection proceeded for 22 h. Base calling used Albacore v4.3.2, which resulted in 899,637 passed reads (N50 length, 7,146 bp), comprising 2,836 Mbp of sequence data.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
