Abstract
ABSTRACTHere, we report the complete genome sequence of Bradyrhizobium sp. strain ORS285, which is able to nodulate Aeschynomene legumes using two distinct strategies that differ in the requirement of Nod factors. The genome sequence information of this strain will help understanding of the different mechanisms of interaction of rhizobia with legumes.
Highlights
We report the complete genome sequence of Bradyrhizobium sp. strain ORS285, which is able to nodulate Aeschynomene legumes using two distinct strategies that differ in the requirement of Nod factors
A draft genome sequence of the ORS285 strain has been previously obtained using 454-pyrosequencing technology, but the presence of a high number of transposase genes and repetitive DNA sequences resulted in a fragmented genome (301 contigs) which made analysis of the regions containing symbiotic genes difficult (5)
De novo assembly of the read sequences was performed using continuous long reads according to the Hierarchical Genome Assembly Process (HGAP) version 3 workflow (DevNet; Pacific Biosciences) as available in the SMRT Analysis software version 2.3.0
Summary
We report the complete genome sequence of Bradyrhizobium sp. strain ORS285, which is able to nodulate Aeschynomene legumes using two distinct strategies that differ in the requirement of Nod factors. A draft genome sequence of the ORS285 strain has been previously obtained using 454-pyrosequencing technology, but the presence of a high number of transposase genes and repetitive DNA sequences resulted in a fragmented genome (301 contigs) which made analysis of the regions containing symbiotic genes difficult (5). We have obtained the complete genome sequence of Bradyrhizobium sp. Strain ORS285 using the Pacific BioSciences (PacBio) sequencing technology.
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