Abstract
The complete and fully annotated genome sequence of a bovine polyomavirus type 1 (BPyV/BEL/1/2014) from aborted cattle was assembled from a metagenomics data set. The 4,697-bp circular dsDNA genome contains 6 protein-coding genes. Bovine polyomavirus is unlikely to be causally related to the abortion cases.
Highlights
From July until November 2014, an increase in the number of bovine abortions showing atypical icteric appearance and splenomegaly was observed in Belgium [1]
In addition to specific diagnostic efforts to diagnose pathogens known to be associated with bovine abortion, a viral metagenomics workflow was applied to samples from 5 aborted fetuses
Bovine polyomavirus type 1 (BPyV-1) sequences were present in all samples and could be confirmed in 5 out of 18 tested samples using real-time RT-PCR [11]
Summary
From July until November 2014, an increase in the number of bovine abortions showing atypical icteric appearance and splenomegaly was observed in Belgium [1]. In addition to specific diagnostic efforts to diagnose pathogens known to be associated with bovine abortion, a viral metagenomics workflow was applied to samples (liver, spleen, placenta, abomasal fluid) from 5 aborted fetuses. Sequencing was performed on a MiSeq sequencer using a MiSeq reagent kit version 3 (Illumina) with 2 ϫ 300-bp paired-end sequencing.
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