Abstract
In the present work, we report the complete genome sequence of Bacillus velezensis DKU_NT_04, isolated from cheonggukjang, which is a traditional Korean fermented soybean paste. The final genome assembly consists of a 4.328-Mbp chromosome with 4,134 coding sequences and a G+C content of 45.21%.
Highlights
In the present work, we report the complete genome sequence of Bacillus velezensis DKU_NT_04, isolated from cheonggukjang, which is a traditional Korean fermented soybean paste
The cleaned reads were de novo assembled using the RS Hierarchical Genome Assembly Process (HGAP) protocol v3.0, short subreads were aligned on Volume 9 Issue 24 e00477-20
Long subreads with Basic Local Alignment with Successive Refinement (BLASR), the assembly was performed with Celera Assembler, and the result was polished with error correction by Quiver v1 in single-molecule real-time (SMRT) Portal 2.3 [9,10,11]
Summary
We report the complete genome sequence of Bacillus velezensis DKU_NT_04, isolated from cheonggukjang, which is a traditional Korean fermented soybean paste. The whole genome of B. velezensis DKU_NT_04 was sequenced with a 20-kb SMRTbell library (PacBio DNA/polymerase binding kit P6) on the RS II sequencing platform (Pacific Biosciences, USA) using C4 chemistry with 8 single-molecule real-time (SMRT) cells at Macrogen (Seoul, Republic of Korea) [8].
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