Abstract
Here, we describe the complete genome assembly of a Bacillus mycoides isolate collected from a boreal forest soil associated with permafrost thaw. Using only long-read Nanopore sequences and VolTRAX library preparation, we assembled two circular contigs totaling 5,789,722 bp (N 50, 5,306,036), a complete chromosome with one plasmid.
Highlights
We describe the complete genome assembly of a Bacillus mycoides isolate collected from a boreal forest soil associated with permafrost thaw
We ran a final polish with medaka v0.6.1, specifying the flipflop model (-m r941_flip) and default parameters
PATRIC assigned the isolate as a member of the Bacillus cereus group, with close identity to the psychrotolerant species Bacillus mycoides
Summary
We describe the complete genome assembly of a Bacillus mycoides isolate collected from a boreal forest soil associated with permafrost thaw. We used 1 g of DNA as input for the VolTRAX V2 (Oxford Nanopore Technologies [ONT]) to prepare a sequencing library (VSK-VSK002 workflow). We base called the raw data using Guppy v2.3.1 (ONT), specifying the flipflop model (– c dna_r9.4.1_450bps_flipflop.cfg) and default parameters (Table 1).
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
