Abstract

Arthrobacter sp. strain FB24 is a species in the genus Arthrobacter Conn and Dimmick 1947, in the family Micrococcaceae and class Actinobacteria. A number of Arthrobacter genome sequences have been completed because of their important role in soil, especially bioremediation. This isolate is of special interest because it is tolerant to multiple metals and it is extremely resistant to elevated concentrations of chromate. The genome consists of a 4,698,945 bp circular chromosome and three plasmids (96,488, 115,507, and 159,536 bp, a total of 5,070,478 bp), coding 4,536 proteins of which 1,257 are without known function. This genome was sequenced as part of the DOE Joint Genome Institute Program.

Highlights

  • Arthrobacter sp. strain FB24 was isolated from a microcosm made from soil collected at an Indiana Department of Transport facility in Seymour, Indiana

  • Based on a summary of genomic features listed on the Integrated Microbial Genomes (IMG) [34] there are 4,536 protein coding sequences identified, of which 3,279 (70.94%, Table 4) have been assigned to a COG functional category (Table 5, Figure 3and Figure 4)

  • The random shotgun method was used in Sanger sequencing the genome of Arthrobacter sp. strain FB24 at the DOE-Joint Genome Institution (DOEJGI)

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Summary

Introduction

Strain FB24 was isolated from a microcosm made from soil collected at an Indiana Department of Transport facility in Seymour, Indiana. Details of microcosm enrichment and isolation procedures used to obtain the Arthrobacter strain have been described previously [2]. Strain FB24 was chosen for sequencing by DOE-JGI because of its extreme resistance to chromate.

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