Abstract

Spores of thermophilic spore-forming bacteria are a common cause of contamination in dairy products. We isolated the thermophilic strain Anoxybacillus flavithermus TNO-09.006 from a milk-processing plant, and we report the complete genome of this isolate consisting of a single chromosome of 2.65 Mb.

Highlights

  • Spores of thermophilic spore-forming bacteria are a common cause of contamination in dairy products

  • We identified three strains with relatively high biofilm-forming capacities and sequenced their genomes: Geobacillus thermoglucosidans TNO-09.020 (1), Anoxybacillus flavithermus TNO-09.006, and Geobacillus stearothermophilus TNO-09.008

  • The genome of A. flavithermus TNO-09.006 consists of a single chromosome (2.65 Mb; 42% GϩC content) in 1 scaffold containing 68 contigs; no plasmids were found

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Summary

Introduction

Spores of thermophilic spore-forming bacteria are a common cause of contamination in dairy products. One of the regular problems in the production of dairy concentrates is contamination by heat-resistant spores from the biofilms of thermophilic bacteria of the genera Anoxybacillus and Geobacillus. We isolated 20 thermophilic species from fouling samples of two dairy-production plants and screened for their ability to form biofilms.

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