Complete Genome Sequence of Aneurinibacillus migulanus E1, a Gramicidin S- and d-Phenylalanyl-l-Propyl Diketopiperazine-Deficient Mutant.

Lassaad Belbahri,Lenka Luptakova,Faizah N Alenezi,Steve Woodward,Mostafa E Rateb

doi:10.1128/genomea.01441-15

Lassaad Belbahri, Lenka Luptakova + Show 3 more

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https://doi.org/10.1128/genomea.01441-15

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Abstract

We report here the complete genome sequence of the Aneurinibacillus migulanus E1 mutant deficient in gramicidin S (GS) and d-phenylalanyl-l-propyl diketopiperazine (DKP) formation. The genome consists of a circular chromosome (6,301,904 bp, 43.20% G+C content) without any plasmid. The complete genome sequence enables further investigation of the biosynthetic mechanism and the biological function of gramicidin S.

Highlights

We report here the complete genome sequence of the Aneurinibacillus migulanus E1 mutant deficient in gramicidin S (GS) and D-phenylalanyl-L-propyl diketopiperazine (DKP) formation
GS-deficient mutants in Aneurinibacillus migulanus have been classified into five different categories
The mutant E1 belongs to the fifth group that contains both a phenylalanine-activating enzyme and a complex of proline, valine, ornithine, and leucineactivating enzymes similar to those of the wild-type strain [6]

Summary

Introduction

We report here the complete genome sequence of the Aneurinibacillus migulanus E1 mutant deficient in gramicidin S (GS) and D-phenylalanyl-L-propyl diketopiperazine (DKP) formation. Gramicidin S (GS) is a cationic cyclic decapeptide with the primary structure [cyclo-(Val-Orn-Leu-D-Phe-Pro)2] [1]. GS is an extremely powerful antibiotic drug against a broad spectrum of both Gram-negative and Gram-positive bacteria and against several pathogenic fungi [2,3,4,5]. GS-deficient mutants in Aneurinibacillus migulanus have been classified into five different categories.

Results

Conclusion