Complete Genome Sequence of Anaeromyxobacter sp. Fw109-5, an Anaerobic, Metal-Reducing Bacterium Isolated from a Contaminated Subsurface Environment.

Chao‐Lung Hwang ,Thomas Brettin ,Lj Hauser ,Susan Lucas ,Alexander S Belieav ,Fe Löeffler ,Eileen Dalin ,Tijana Glavina Del Rio ,David Sims ,Frank Larimer ,Athanasios Lykidis ,Alla Lapidus ,Paul Richardson ,Kerrie Barry ,C.s Han ,Alex Copeland ,Jeremy Schmutz ,Jc Detter ,Ra Sanford ,Hope Tice ,Dc Bruce ,Sam Pitluck ,Nikos C Kyrpides ,M C Land ,Matthew W Fields

doi:10.1128/genomea.01449-14

Abstract

We report the genome sequence of Anaeromyxobacter sp. Fw109-5, isolated from nitrate- and uranium-contaminated subsurface sediment of the Oak Ridge Integrated Field-Scale Subsurface Research Challenge (IFC) site, Oak Ridge Reservation, TN. The bacterium’s genome sequence will elucidate its physiological potential in subsurface sediments undergoing in situ uranium bioremediation and natural attenuation.

Highlights

The Oak Ridge Integrated Field-Scale Subsurface Research Challenge (IFC) site encompasses a U(VI)-contaminated area for conducting in situ bioremediation field research
Fw109-5 was isolated from Area 3, further evolutionary distance analysis indicated that sequences detected in the ethanol biostimulation treatment zone belonged to another distinct cluster of Anaeromyxobacter populations, whereas Anaeromyxobacter sp
These results demonstrated that a diverse Anaeromyxobacter population exists at the IFC site and comparative genome analysis provides relevant information about their physiological capacity, which is crucial for future bioremediation designs

Summary

Introduction

The Oak Ridge Integrated Field-Scale Subsurface Research Challenge (IFC) site encompasses a U(VI)-contaminated area for conducting in situ bioremediation field research. Bioremediation efforts at the Area 3 site involved treatment of contaminated groundwater to optimize subsurface conditions for U(VI) reduction by indigenous microorganisms upon ethanol biostimulation [1]. Concurrent cultivation efforts led to isolation of the anaerobic bacterium, Anaeromyxobacter sp. Fw109-5, from a ferric iron enrichment of subsurface sediments collected from monitoring well Fw109 located outside the treatment zone.

Results

Conclusion