Complete Genome Sequence of an N-Acyl Homoserine Lactone Producer, Breoghania sp. Strain L-A4, Isolated from Rhizosphere of Phragmites australis in a Coastal Wetland.

Changfei He,Li Zheng,Yu Ding,Jinfeng Ding,Wendan Chi,Wei Gao,Steven R Gill

doi:10.1128/mra.01539-18

Abstract

The Breoghania sp. strain L-A4 was isolated from the rhizosphere of Phragmites australis in the Qinhaungdao coastal wetland in China. Here, we present the complete genome sequence of strain L-A4, which consists of a chromosome of 5,029,620 bp with a G+C content of 64.53% and 4,964 coding DNA sequences. This strain was the first detected to produce N-acyl homoserine lactone (AHL) signals in a member of this genus.

Highlights

The Breoghania sp. strain L-A4 was isolated from the rhizosphere of Phragmites australis in the Qinhaungdao coastal wetland in China
We performed complete genome sequencing of L-A4 and hope to confirm the ecological functions regulated by the quorum sensing (QS) system
The 10-kb SMRTbell library was constructed with purification DNA fragments of AMPure PB magnetic beads [9]

Summary

Introduction

The Breoghania sp. strain L-A4 was isolated from the rhizosphere of Phragmites australis in the Qinhaungdao coastal wetland in China. Strain L-A4 is a long rod-shaped aerobic bacterium, approximately 0.2 to 0.4 ␮m wide and 0.8 to 1.2 ␮m long (Fig. 1A). One AHL signal, N-hexanoyl-Lhomoserine lactone (C6), can be produced in Breoghania sp. Strain L-A4 and was detected with the thin-layer chromatography (TLC) overlay method using the Agrobacterium tumefaciens KYC55 biosensor method [7, 8] (Fig. 1B). We performed complete genome sequencing of L-A4 and hope to confirm the ecological functions regulated by the QS system.

Results

Conclusion