Complete Genome Sequence of &amp;lt;i&amp;gt;Bacillus thuringiensis&amp;lt;/i&amp;gt; Serovar &amp;lt;i&amp;gt;coreanensis&amp;lt;/i&amp;gt; ST7 with Toxicity to Human Cancer Cells

Jing Zhang,Baoli Zhang,Yiping Liu,Rui Liu,Jun Zhu,Xu Liu

doi:10.4236/aim.2020.1012048

Abstract

Bacillus thuringiensis (Bt) parasporal crystal proteins were well known to be toxic to certain insects and cytocidal activity against various human cancer cells. Bt serovar coreanensis ST7, non-pathogenic to insects and non-hemolytic, has an important parasporin, PS4Aa1 (Cry45Aa1), with potential toxicity to human cancer cells. In this study, we reported the feature of complete genome sequence and the cluster of orthologous groups of proteins function classification of ST7. Meanwhile, the evolutionary of ST7 was also studied. The genome data of ST7 will strongly contribute to a better understanding of the genomic diversity and evolution, and enrich the Bt genome database.

Highlights

Bacillus thuringiensis (Bt) is a typical aerobic, Gram-positive bacterium
Bacillus thuringiensis (Bt) parasporal crystal proteins were well known to be toxic to certain insects and cytocidal activity against various human cancer cells
We reported the feature of complete genome sequence and the cluster of orthologous groups of proteins function classification of ST7

Summary

Introduction

Bacillus thuringiensis (Bt) is a typical aerobic, Gram-positive bacterium. During sporulation, Bt produces one or more parasporal crystal proteins [1]. The Cry45-type contained in the strain ST7 has 100% amino acid sequence homology with Cry45Aa1 of Bt serovar strain A1470 [6], so that the strain ST7 may have toxicity to the same kinds of human cancer cells. Thousands of Bt strains have been isolated, but only a few of them have high toxicity to human cancer cells, and have been not obtained the complete genome sequence [4] [5]. This is the first complete genome sequence of Bt with potential toxicity to human cancer. The analysis of complete genome sequence ofST7 may help in the development of strategies to cope with cancer cells in the medical field

Genome Sequencing and Assembly

Genome Properties

Genome Annotation

Conflicts of Interest