Abstract
Acinetobacter baumannii ATCC 19606T, which is often used in genetic studies as a routine model microorganism, belongs to sequence type 52 (ST52), showing beta-lactam resistance. We present the complete 3.996-Mbp genome sequence (1 chromosome plus 2 plasmids), generated by combining long-read (MinION) and short-read (MiniSeq) sequencing data.
Highlights
Acinetobacter baumannii ATCC 19606T, which is often used in genetic studies as a routine model microorganism, belongs to sequence type 52 (ST52), showing beta-lactam resistance
The complete genome sequence was constructed as a total sequence of 3,995,851 bp, with a mean GϩC content of 39.2%
All 452 BUSCOs were located in the assembled genome
Summary
Acinetobacter baumannii ATCC 19606T, which is often used in genetic studies as a routine model microorganism, belongs to sequence type 52 (ST52), showing beta-lactam resistance. Reads), which were quality filtered using Trimmomatic v0.30 (quality scores of Ն30) (6), to correct sequencing errors and to detect single nucleotide polymorphisms (SNPs) and indels using Pilon v1.20.1 (7) twice with binary alignment map (BAM) files generated from MinION and Illumina reads. Using Minimap 2 v2.17 (8), 99.7% of MiniSeq reads were mapped onto the final assembly, and the final genome coverage was 125ϫ.
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