Complete Genome Sequence of a Virulent Leptospira interrogans Serovar Copenhageni Strain, Assembled with a Combination of Nanopore and Illumina Reads.

Alejandro Llanes,Sreekumari Rajeev,Dhani Prakoso,Carlos Mario Restrepo,Irene L G Newton

doi:10.1128/mra.00200-20

Abstract

Here, we present the complete genome sequence of a highly virulent Leptospira interrogans serovar Copenhageni strain isolated from a dog with severe leptospirosis. In this work, a gapless genome draft was assembled with a combination of Nanopore and Illumina data of relatively low coverage.

Highlights

We present the complete genome sequence of a highly virulent Leptospira interrogans serovar Copenhageni strain isolated from a dog with severe leptospirosis
The number of suspected errors decreased from 14,672 (0.3%) in the original Canu assembly to 92 (0.002%) after polishing with Pilon
At least 11 pseudogenes from Fiocruz L1-130 appear to have functional orthologs in the genomes of strains SK1 and 56601, despite the latter belonging to L. interrogans serovar Lai

Summary

Introduction

We present the complete genome sequence of a highly virulent Leptospira interrogans serovar Copenhageni strain isolated from a dog with severe leptospirosis. Illumina and Nanopore sequencing generated 1.1 million 300-bp paired-end reads and 142,300 reads with a median length of 8 kb, respectively.

Results

Conclusion