Abstract
ABSTRACTWe report here the complete genome sequence of a Citrus tristeza virus (CTV) from Uruguay, sequenced by using Illumina and Sanger sequencing technology. This CTV DSST-17 genome clustered within genotype resistance breaking (RB) and presents two recombination events.
Highlights
Citrus crops are among the most important commercial fruit crops worldwide
The complete genome obtained was compared with all available full genomes of the GenBank database using MEGA 6 [8]
Phylogenetic analysis grouped the DSST-17 isolate within the resistance breaking (RB) genotype with a genome nucleotide identity ranging from 94.1% to 99.6%
Summary
Citrus crops are among the most important commercial fruit crops worldwide. Citrus tristeza virus (CTV) (Closterovirus, Closteroviridae) is one of the most destructive pathogens that affects citrus trees around the world and has been responsible for the loss of over 100 million trees in the past 70 years [1]. Subisolate DSST-17, obtained by single aphid transmission from a field sample collected in 2014 in Salto, Uruguay, from a Navelina sweet orange, was subjected to Illumina sequencing technology. Total RNA was extracted using the RNeasy plant minikit (Qiagen) and submitted to RNA library preparation with a NEBNext Ultra II RNA library prep kit (Illumina). The library was sequenced using the NextSeq 500 system platform (Illumina).
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