Abstract
A polycyclic aromatic hydrocarbon-degrading bacterium designated strain Ca6, a member of the family Rhodocyclaceae and a representative of the uncharacterized pyrene group 1 (PG1), was isolated and its genome sequenced. The presence of several genes suspected to be associated with PG1 was confirmed, and additional genes for aromatic compound metabolism were detected.
Highlights
A polycyclic aromatic hydrocarbon-degrading bacterium designated strain Ca6, a member of the family Rhodocyclaceae and a representative of the uncharacterized pyrene group 1 (PG1), was isolated and its genome sequenced
A previously constructed metagenome from a mixed culture dominated by PG1 contained eight different ring-hydroxylating dioxygenase (RHD) genes predicted to code for enzymes involved in the initial step of aerobic Polycyclic aromatic hydrocarbons (PAHs) metabolism [5]
Six of those RHDs heterologously expressed in Escherichia coli demonstrated PAH transformation capabilities [5]
Summary
A polycyclic aromatic hydrocarbon-degrading bacterium designated strain Ca6, a member of the family Rhodocyclaceae and a representative of the uncharacterized pyrene group 1 (PG1), was isolated and its genome sequenced. Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous chemicals that, when concentrated at contaminated sites, can pose a serious threat to human and environmental health. Of particular concern are high-molecular-weight PAHs of four or more aromatic rings that are potentially both more carcinogenic and resistant to microbial degradation than are their low-molecularweight counterparts [1, 2]. A prior stable-isotope probing study with the four-ring PAH pyrene revealed DNA sequences from several groups of uncharacterized proteobacteria [3].
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