Abstract
Genomic sequencing for early identification of Ebola virus remains a big challenge in low-income countries. Here, we report the complete genome sequence of an Ebola virus strain obtained during the 2017 Likati outbreak in the Democratic Republic of the Congo (DRC) by using the Oxford Nanopore Technologies (ONT) MinION sequencer.
Highlights
Genomic sequencing for early identification of Ebola virus remains a big challenge in low-income countries
Reverse transcription-PCR (RT-PCR) was performed with each of the EBOV-specific primer pools kindly provided by the ARTIC project (2) to amplify the EBOV genome
The purified DNA was quantified on a Qubit 1.0 fluorimeter (Thermo Fisher Scientific), and libraries were prepared using the 1D genomic DNA by ligation (SQK-LSK108) kit and the protocol supplied by Oxford Nanopore Technologies (ONT)
Summary
Genomic sequencing for early identification of Ebola virus remains a big challenge in low-income countries. We present the complete genome sequence of the EBOV strain found in one of these two samples, obtained using the MinION sequencer. Reverse transcription-PCR (RT-PCR) was performed with each of the EBOV-specific primer pools kindly provided by the ARTIC project (2) to amplify the EBOV genome.
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