Complete Genome Sequence of a Betanodavirus Isolated from Half-Smooth Tongue Sole (Cynoglossus semilaevis).

Jin Li,Sheng Qiang Wang,Jie Huang,Zi Dan Su,Wei Guang Geng,Cheng Yin Shi

doi:10.1128/genomea.01312-14

Abstract

Betanodavirus, commonly called nervous necrosis virus (NNV) of fish, has emerged as a major constraint on marine aquaculture worldwide. Here, we report the complete genome sequence of a betanodavirus (strain CsCN128) isolated from diseased half-smooth tongue sole (Cynoglossus semilaevis) in China. The genome sequence of strain CsCN128 shares ≥98.7% similarity with seven-band grouper nervous necrosis virus from Japan. Phylogenetic analysis indicates that strain CsCN128 belongs to the red-spotted grouper nervous necrosis virus (RGNNV) genotype of betanodavirus. The genome of the strain CsCN128 will facilitate further study on the molecular epidemiology and natural susceptible host range of betanodaviruses.

Highlights

Betanodavirus, commonly called nervous necrosis virus (NNV) of fish, has emerged as a major constraint on marine aquaculture worldwide
In order to determine the molecular characterization of strain CsCN128, we sequenced the complete genome of the virus
Based on known betanodavirus nucleotide sequences, two pairs of reverse transcription-PCR (RT-PCR) primers were selected for amplification of the middle fragments of the strain CsCN128 genome (RNA1 and RNA2, respectively) [4, 5]

Summary

Introduction

Betanodavirus, commonly called nervous necrosis virus (NNV) of fish, has emerged as a major constraint on marine aquaculture worldwide. Betanodavirus, commonly called nervous necrosis virus (NNV) of fish, is a major causative agent of disease in marine fish. Halfsmooth tongue sole (Cynoglossus semilaevis) has not been described as the host of betanodavirus up to now. In August 2012, an outbreak of disease caused by C. semilaevis broke out in a hatchery in northern China.

Results

Conclusion