Abstract
BackgroundWe have previously isolated Bacillus subtilis HMNig-2 and MENO2 strains, from honey and the honeybee gut microbiome respectively, and demonstrated these strains to produce levansucrase with potential probiotics characteristics. Here we report their complete genome sequence and comparative analysis with other and other B. subtilis strains. ResultsThe complete genome sequences of Bacillus subtilis HMNig-2 and MENO2 were de novo assembled from MiSeq paired-end sequence reads and annotated using the RAST tool. Whole-genome alignments were performed to identify functional differences associated with their potential use as probiotics. ConclusionsThe comparative analysis and the availability of the genome sequence of these two strains will provide comprehensive analysis about the diversity of these valuable Bacillus strains and the possible impact of the environment on bacterial evolution. Significance and impact of studyWe introduce complete genome sequence of two new B. subtilis strains HMNig-2 and MENO2 with probiotic potential and as cell factories for the production of levan and other valuable components for pharmaceutical and industrial applications
Highlights
We have previously isolated Bacillus subtilis HMNig-2 and MENO2 strains, from honey and the honeybee gut microbiome respectively, and demonstrated these strains to produce levansucrase with potential probiotics characteristics
Significance and impact of study: We introduce complete genome sequence of two new B. subtilis strains HMNig-2 and MENO2 with probiotic potential and as cell factories for the production of levan and other valuable components for pharmaceutical and industrial applications
Even though soil is considered the principal habitat for Bacillus species, Hamdy et al Journal of Genetic Engineering and Biotechnology (2020) 18:34 they have been detected in honey [6] and the feces of bee larvae [7, 8]
Summary
We have previously isolated Bacillus subtilis HMNig-2 and MENO2 strains, from honey and the honeybee gut microbiome respectively, and demonstrated these strains to produce levansucrase with potential probiotics characteristics. We report their complete genome sequence and comparative analysis with other and other B. subtilis strains. Bacillus strains have been selected for use in probiotic supplements for human and animal consumption [9,10,11,12] As these bacteria form spores, they present advantages over other common probiotic species with the ability to tolerate high temperatures and sustain viability for long periods without the need for cooling or freezing [12, 13]. Bacillus species are widely used in industrial biotechnology as cell factories to produce a multiplicity of metabolites including antibiotics, bioinsecticides, enzymes, and lipopeptides [14,15,16,17]
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