Complete Genome Sequence and Annotation of a Campylobacter jejuni Strain, MTVDSCj20, Isolated from a Naturally Colonized Farm-Raised Chicken.

Michael E Taveirne,Craig T Parker,Drew T Dunham,William G Miller,Steven Huynh,Victor J Dirita

doi:10.1128/genomea.00852-14

Abstract

Campylobacter jejuni is a major cause of human food-borne illness, with contaminated poultry products serving as a main source of human infection. C. jejuni strain MTVDSCj20 was isolated from the cecal contents of a farm-raised chicken that was naturally colonized with Campylobacter. We present here the complete annotated genome sequence of MTVDSCj20.

Highlights

Campylobacter jejuni is a major cause of human food-borne illness, with contaminated poultry products serving as a main source of human infection
The colonies that arose on selective medium were confirmed to be Campylobacter by multiplex PCR using primers CJF/R (C. jejuni hipO), CCF/R (C. coli glyA), and 23SF/R (C. jejuni 23s rRNA) [7]
A reference assembly against the C. jejuni NCTC 11168 genome was performed within Newbler

Summary

Introduction

Campylobacter jejuni is a major cause of human food-borne illness, with contaminated poultry products serving as a main source of human infection. Campylobacter jejuni is a leading cause of bacterially derived human food-borne illness worldwide [1, 2]. Human Campylobacter-derived gastroenteritis, campylobacteriosis, is predominantly caused by two species of Campylobacter, C. jejuni and C. coli [3, 4]. Campylobacter infections are usually due to the consumption of contaminated poultry products, mainly chicken [5].

Results

Conclusion