Abstract
Although more than 100 genome sequences of Pasteurella multocida are available, comprehensive and complete genome sequence analysis is limited. This study describes the analysis of complete genome sequence and pathogenomics of P. multocida strain PMTB2.1. The genome of PMTB2.1 has 2176 genes with more than 40 coding sequences associated with iron regulation and 140 virulence genes including the complete tad locus. The tad locus includes several previously uncharacterized genes such as flp2, rcpC and tadV genes. A transposable phage resembling to Mu phages was identified in P. multocida that has not been identified in any other serotype yet. The multi-locus sequence typing analysis assigned the PMTB2.1 genome sequence as type ST101, while the comparative genome analysis showed that PMTB2.1 is closely related to other P. multocida strains with the genomic distance of less than 0.13. The expression profiling of iron regulating-genes of PMTB2.1 was characterized under iron-limited environment. Results showed significant changes in the expression profiles of iron-regulating genes (p < 0.05) whereas the highest expression of fecE gene (281 fold) at 30 min suggests utilization of the outer-membrane proteins system in iron acquisition at an early stage of growth. This study showed the phylogenomic relatedness of P. multocida and improved annotation of important genes and functional characterization of iron-regulating genes of importance to the bacterial growth.
Highlights
Pasteurella multocida (PM) species of the genus Pasteurella are Gram-negative facultative anaerobic bacteria belonging to the family Pasteurellaceae [1]
The P. multocida strain PMTB2.1 was isolated from a buffalo that died of septicemia in Malaysia [20] and was identified by species-specific PCR [7,19]
The circularity of the chromosome was confirmed by PCR where the trimmed sequences were successfully aligned with the complete genome sequence of PMTB2.1 in such a way that the first 1,538 bp at the end of circle aligned with the last nucleotides at position
Summary
Pasteurella multocida (PM) species of the genus Pasteurella are Gram-negative facultative anaerobic bacteria belonging to the family Pasteurellaceae [1]. Based on its capsular antigen, the P. multocida is grouped into A, B, D, E and F capsular types and further classified into 16 serotypes (1–16) based on lipopolysaccharide (LPS) antigen [2,3]. P. multocida was earlier identified as a commensal in the upper respiratory tract of mammals and birds. P. multocida is often associated with acute as well. Genes 2019, 10, 81 as chronic infections in avian and bovine that can lead to significant morbidity and mortality [4,5]. Atrophic rhinitis in pigs and pasteurellosis characterized as respiratory diseases namely pneumonia and hemorrhagic septicemia (HS) in cattle and buffaloes are the most important veterinary diseases [6,7]. Pasteurellosis caused by P. multocida is an acute septicemic disease characterized by high morbidity and is a high-impact disease in livestock, according to the World
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
