Complete genome of Pseudomonas chlororaphis strain UFB2, a soil bacterium with antibacterial activity against bacterial canker pathogen of tomato.

Peng Deng,Sonya M Baird,Xiaoqiang Wang,Shi-En Lu

doi:10.1186/s40793-015-0106-x

Peng Deng, Sonya M Baird + Show 2 more

Open Access

https://doi.org/10.1186/s40793-015-0106-x

Copy DOI

Journal: Environmental microbiome	Publication Date: Dec 1, 2015
Citations: 76	License type: CC BY 4.0

Affiliation: Mississippi State University

Abstract

Strain UFB2 was isolated from a soybean field soil in Mississippi and identified as a member of Pseudomonas chlororaphis. Strain UFB2 has a broad-spectrum antimicrobial activity against common soil-borne pathogens. Plate assays showed that strain UFB2 was especially efficient in inhibiting the growth of Clavibacter michiganensis 1–07, the causal agent of the devastating bacterial canker of tomato. Here, the complete genome sequence of P. chlororaphis strain UFB2 is reported and described. The strain UFB2 genome consists of a circular chromosome of 6,360,256 bp of which 87.86 % are protein-coding bases. Genome analysis revealed multiple gene islands encoding various secondary metabolites such as 2,4-diacetylphloroglucinol. Further genome analysis will provide more details about strain UFB2 antibacterial activities mechanisms and the use of this strain as a potential biocontrol agent.

Highlights

Bacterial strains of Pseudomonas chlororaphis are aerobic Gram-positive bacteria and many of the strains possess a wide-spectrum antifungal activity against soil-borne plant pathogens [1,2,3,4,5]
The use of P. chlororaphis strains as biocontrol agents is promising because they are capable of producing a variety of antimicrobial secondary metabolites including phenazine-1-carboxamide, 2-hydroxyphenazine, pyrrolnitrin, hydrogen cyanide, chitinases and proteases [6,7,8]
The complete genome sequence of P. chlororaphis strain UFB2 is described in this report

Summary

Introduction

Bacterial strains of Pseudomonas chlororaphis are aerobic Gram-positive bacteria and many of the strains possess a wide-spectrum antifungal activity against soil-borne plant pathogens [1,2,3,4,5]. The whole genomes of a few P. chlororaphis strains that exhibit antifungal activity have been sequenced. Genome sequences of P. chlororaphis strains with significant antibacterial activity have not been reported previously. Plate assays indicated P. chlororaphis strain UFB2 has a broad spectrum of antimicrobial activities, especially against bacterial canker pathogen of tomato: Clavibacter michiganensis [12, 13].

Results

Conclusion