Abstract
Bacteriophage Pookie is a novel podophage, isolated from soil, which infects Bacillus megaterium. B. megaterium is an important host for large-scale recombinant protein production. Here, we present the complete genome of phage Pookie and describe its core features.
Highlights
Bacteriophage Pookie is a novel podophage, isolated from soil, which infects Bacillus megaterium
Bacillus megaterium is a Gram-positive bacteria widely used in industry due to its efficient protein secretion system and ability to grow on various inexpensive carbon sources (1)
Bacteriophages infecting B. megaterium are readily found in the environment and may be able to provide biotechnological advances
Summary
Bacteriophage Pookie is a novel podophage, isolated from soil, which infects Bacillus megaterium. Bacteriophages infecting B. megaterium are readily found in the environment and may be able to provide biotechnological advances. We describe here the genome of novel B. megaterium podophage Pookie. Phage DNA was sequenced in an Illumina MiSeq 250-bp paired-end run with a 550-bp insert library at the Genomic Sequencing and Analysis Facility at the University of Texas (Austin, TX).
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