Abstract
Lactococcus petauri CF11 was originally isolated from the gut of healthy humans. To determine the underlying molecular and genetic mechanisms of the probiotic potential of CF11, we performed complete genome sequencing, annotation, and comparative genome analysis. The complete genome of L. petauri CF11 comprised of 1,997,720 bp, with a DNA G+C content of 38.21 mol% containing 1982 protein coding genes and 16 rRNA operons. We found that 1206 genes (56.05%) were assigned a putative function using the gene ontology (GO) resource. The gene products of CF11 were primarily concentrated in molecular function and biological processes, such as catalysis, binding, metabolism, and cellular processes. Furthermore, 1,365 (68.87%) genes were assigned an illative function using COGs. CF11 proteins were associated with carbohydrate transport and metabolism, and amino acid transport and metabolism. This indicates that CF11 bacteria can perform active energy exchange. We classified 1,111 (56.05%) genes into six KEGG functional categories; fructose-bisphosphate aldolase and the phosphoenol pyruvate:phosphotransferase system (PTS), which are necessary in producing short-chain fatty acids (SCFAs), were excited in the carbohydrate metabolic pathway. This suggests that L. petauri CF11 produces SCFAs via glycolysis. The genomic island revealed that some regions contain fragments of antibiotic resistance and bacteriostatic genes. In addition, ANI analysis showed that L. petauri CF11 had the closest relationship with L. petauri 159469T, with an average nucleotide consistency of 98.03%. Taken together, the present study offers further insights into the functional and potential role of L. petauri CF11 in health care.
Highlights
The intestinal tract is the largest microecosystem in the human body
We obtained the genome of L. petauri CF11, which was comprised of 1 contig consisting of 1,997,720 bp, with an average G+C content of 38.21 mol% of the genome (Table 1)
A genomic island was identified in the genome of L. petauri CF11, which was related to the bacterial fitness and virulence (Wu et al, 2014)
Summary
The intestinal tract is the largest microecosystem in the human body. It contains a significant number of intestinal microbes, which are known as the intestinal flora. Short Illumina reads from second-generation data are used to assist the correction of the long-read third-generation data to improve the accuracy of genome assembly without increasing the cost of sequencing. The hybrid assembly genome was obtained based on short Illumina reads and long Pac Bio reads. These data offer a good foundation for the future research on genome function annotation, comparative genome analysis, and re-sequencing
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