Abstract
ABSTRACTThe complete genome sequences of Aureimonas sp. strain SA4125 and its native plasmid pSA4125 were determined. The genome sequence comprises 4,968,066 bp, with a GC content of 66.0%, and contains 4,691 coding DNA sequences (CDSs), 3 rRNA operons, and 50 tRNAs. The native plasmid comprises 131,777 bp, with a GC content of 62.3%, and contains 138 CDSs.
Highlights
Members of the genus Aureimonas are aerobic, Gram stain-negative, rod-shaped, motile, and aerobic bacteria having Q-10 as the dominant isoprenoid quinone [1]
For DNA extraction, SA4125 was cultivated in modified Brock’s basal salt (MBS) and 0.1% yeast extract at 20°C; 500 ml of log-phase culture was centrifuged (8,000 Â g, 4°C, 15 min)
For long-read sequencing, a DNA library was prepared following the protocol as described by Oxford Nanopore Technologies (ONT) (NBE_9065_v109_revZ_ 14Aug2019) and applied to a MinION sequencer with a R9 flow cell
Summary
Complete Genome and Plasmid Sequences of the Psychrotolerant Aureimonas Strain SA4125, Isolated from Antarctic Moss Vegetation. Sakai,a Ryo Matsuda,b Satoshi Imura,c,d Norio Kurosawaa,b aDepartment of Science and Engineering for Sustainable Innovation, Faculty of Science and Engineering, Soka University, Hachioji, Tokyo, Japan bDepartment of Environmental Engineering for Symbiosis, Graduate School of Science and Engineering, Soka University, Hachioji, Tokyo, Japan cNational Institute of Polar Research, Research Organization of Information and Systems, Tokyo, Japan dDepartment of Polar Science, School of Multidisciplinary Sciences, The Graduate University for Advanced Studies, SOKENDAI, Tokyo, Japan
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