Complete genome analysis ofPseudomonas furukawaiiZS1 isolated from grass carp (Ctenopharyngodon idellus) culture water.

Abstract

Pseudomonas furukawaii ZS1, isolated from grass carp (Ctenopharyngodon idellus) culture water, exhibits efficient aerobic nitrate reduction without nitrite accumulation; however, the molecular pathway underlying this aerobic nitrate reduction remains unclear. In this study, we constructed a complete genome map of P. furukawaii ZS1 and performed a comparative genomic analysis with a reference strain. The results showed that P. furukawaii ZS1 genome was 6,026,050 bp in size and contained 5,427 predicted protein-coding sequences. The genome contained all the necessary genes for the dissimilatory nitrate reduction to ammonia pathway, but lacked those for the assimilatory nitrate reduction pathway. Simultaneously, the genome also contained genes that converted ammonia to organic nitrogen. The putative genes associated with the nitrogen and oxidative phosphorylation pathways implied that ZS1 can perform respiration and nitrate reduction simultaneously under aerobic conditions. This work provided insights at the molecular level on the nitrogen metabolic pathways in Pseudomonas species.