Complete genome analysis of the newly isolated Shigella sonnei phage vB_SsoM_Z31.

Bingdong Wei,Long Chen,Wei Yu,Cong Cong,Lichun Zhang,Lin Zheng,Yongping Xu

doi:10.1007/s00705-021-05121-y

Abstract

This work describes the characterization and genome annotation of the newly isolated lytic phage vB_SsoM_Z31 (referred to as Z31), isolated from wastewater samples collected in Dalian, China. Transmission electron microscopy revealed that phage Z31 belongs to the family Myoviridae, order Caudovirales. This phage specifically infects Shigella sonnei, Shigella dysenteriae, and Escherichia coli. The genome of the phage Z31 is an 89,355-bp-long dsDNA molecule with a G+C content of 38.87%. It was predicted to contain 133 ORFs and encode 24 tRNAs. No homologs of virulence factor genes or antimicrobial resistance genes were found in this phage. Based on the results of nucleotide sequence alignment and phylogenetic analysis, phage Z31 was assigned to the genus Felixounavirus, subfamily Ounavirinae.

Highlights

Shigella species are Gram-negative, nonmotile bacilli belonging to the family Enterobacteriaceae
Spot test indicated that five Shigella strains tested and Enterotoxigenic E. coli K88 CVCC 83902 were lysed by the phage Z31 (Table 1)
Efficiency of plating (EOP) results revealed that phage Z31 was showed high infection efficiency against four Shigella strains (S. sonnei BNCC 192105 EOP=0.98, S.sonnei BNCC 108852 EOP=0.88, S. dysenteriae CGMCC 10983 EOP=0.68, and S. dysenteriae BNCC 103609 EOP=0.94) and medium infection efficiency against Enterotoxigenic E. coli K88 (EOP=0.41)

Summary

Introduction

Shigella species are Gram-negative, nonmotile bacilli belonging to the family Enterobacteriaceae. This work describes the characterization and genome annotation of a newly isolated lytic phage vB_SsoM_Z31 (referred to as Z31), isolated from wastewater samples collected in Dalian, China. It has been predicted to contain 133 ORFs, and 24 tRNAs. No homologs of virulence factors or antimicrobial resistance genes were found in this phage.

Results

Conclusion