Complete degradation of azo dye acid red 337 by Bacillus megaterium KY848339.1 isolated from textile wastewater

Abstract

ABSTRACT Biodegradation has been proven as the most efficient, eco-friendly and cost-effective technique for the removing of complex organic matters such as textile dyes from wastewater effluents. Some environmentally friend bacterial strains play an important role in such field. Acid red 337, an azo dye used extensively in textile industry, was reported as hazardous recalcitrant, when released into the aqueous environment. In the present research, a potential bacterial strain, capable of degrading acid red 337 (AR 337) dye was isolated from a textile wastewater effluent. Using 16S rRNA sequence analysis, the bacterium was identified as Bacillus megaterium KY848339.1. The decolorization capability of B. megaterium for AR 337 dye was optimized; the bacterium could remove 91% of dye concentration of 500 mg L−1 within 24 h when the inoculum size was 10% wt./v, solution pH was 7 and the incubation temperature was 30°C. The Liquid Chromatography-Mass Spectrum (LC-MS) analysis indicated the degradation of AR 337 azo dye by B. megaterium, to small aliphatic compounds and CO2. The application of B. megaterium on wastewater contaminated with red dyes using 10% wt./v of bacterial cells concentration was resulted in 98.9% removing of red color through 10 days.