Abstract
Wolbachia, a gram-negative mathrm{alpha }-proteobacterium, is an endosymbiont found in some arthropods and nematodes. Diaphorina citri Kuwayama, the vector of ‘Candidatus Liberibacter asiaticus’ (CLas), are naturally infected with a strain of Wolbachia (wDi), which has been shown to colocalize with the bacteria pathogens CLas, the pathogen associated with huanglongbing (HLB) disease of citrus. The relationship between wDi and CLas is poorly understood in part because the complete genome of wDi has not been available. Using high-quality long-read PacBio circular consensus sequences, we present the largest complete circular wDi genome among supergroup-B members. The assembled circular chromosome is 1.52 megabases with 95.7% genome completeness with contamination of 1.45%, as assessed by checkM. We identified Insertion Sequences (ISs) and prophage genes scattered throughout the genomes. The proteins were annotated using Pfam, eggNOG, and COG that assigned unique domains and functions. The wDi genome was compared with previously sequenced Wolbachia genomes using pangenome and phylogenetic analyses. The availability of a complete circular chromosome of wDi will facilitate understanding of its role within the insect vector, which may assist in developing tools for disease management. This information also provides a baseline for understanding phylogenetic relationships among Wolbachia of other insect vectors.
Highlights
IntroductionA gram-negative α-proteobacterium, is an endosymbiont found in some arthropods and nematodes
Wolbachia, a gram-negative α-proteobacterium, is an endosymbiont found in some arthropods and nematodes
The genome sequence of the Wolbachia culture isolated from D. citri was completely assembled and compared with other Wolbachia genomes available in the NCBI database
Summary
A gram-negative α-proteobacterium, is an endosymbiont found in some arthropods and nematodes. CLas interacts with host D. citri and its endosymbionts, including Wolbachia, a gram-negative α-proteobacteria[3,4,5]. Liberibacter asiaticus which is critical for the survival of both endosymbionts in the psyllid[5] This suggests a potential role of Wolbachia in CLas transmission and underscores the need for a well characterized Wolbachia genome[4] in gaining a better grasp of and combating this dreadful citrus disease. In some arthropods, such as Drosophila melanogaster, Aedes aegypti, Culex pipiens, Acraea encedon, Armadillidium vulgare, and Asobara tabida, Wolbachia can alter host reproduction and increase viral resistance[7,8,9]. The genome dataset will enhance our ability to elucidate the interactions of wDi with its D. citri host and associated endosymbionts
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