Abstract
The full genome sequences of two isolates of bluetongue virus (BTV) from a commercial sheeppox vaccine were determined. Strain SPvvvv/02 shows low sequence identity to its closest relative, strain BTV-26 KUW2010/02, indicating the probable detection of a novel BTV genotype, whereas strain SPvvvv/03 shows high sequence identity to strain BTV-28/1537/14.
Highlights
The full genome sequences of two isolates of bluetongue virus (BTV) from a commercial sheeppox vaccine were determined
Total RNA was extracted from the cell pellets using TRIzol reagent (Life Technologies, UK)
99.9 99.9 100.0 100.0 99.9 100.0 100.0 100.0 99.9 100.0 a The entire genome of SPvvvv/02 has a segment length of 19,181 bp, 790,759 mapped reads, and an average coverage depth of 4,824 bp, and the entire genome of SPvvvv/03 has a segment length of 19,193 bp, 1,960,587 mapped reads, and an average coverage depth of 12,448 bp. b BTV segment 10 (GenBank accession number KT946752) was first sequenced directly using RNA extracted from a commercial sheeppox vaccine [8]; it had 100% nucleotide identity with SPvvvv/02 and 98.39% identity with both SPvvvv/03 and BTV-28/1537/14
Summary
The full genome sequences of two isolates of bluetongue virus (BTV) from a commercial sheeppox vaccine were determined. The commercial sheeppox vaccine (JOVAC, batch number 200214/01) was resuspended in 1 ml phosphate-buffered saline (PBS; pH 7.20) and used as the inoculum for virus propagation in baby hamster kidney (BHK) cells. Library preparation was performed using the Nextera XT DNA library kit (Illumina, USA), and paired-end read sequencing (2 ϫ 150 bp) was carried out using an Illumina MiSeq instrument.
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