Complete, Closed Genome Sequences of 10 Salmonella enterica subsp. enterica Serovar Typhimurium Strains Isolated from Human and Bovine Sources.

Scott V Nguyen,James L Bono,Gregory P Harhay,Patricia I Fields,Joseph M Bosilevac,Christy M Kelley,Monica Santovenia,Blake A Dinsmore,Dayna M Harhay,Timothy P L Smith,Rong Wang

doi:10.1128/genomea.01212-16

Abstract

Salmonella enterica is a leading cause of enterocolitis for humans and animals. S. enterica subsp. enterica serovar Typhimurium infects a broad range of hosts. To facilitate genomic comparisons among isolates from different sources, we present the complete genome sequences of 10 S. Typhimurium strains, 5 each isolated from human and bovine sources.

Highlights

Genomic DNA was purified with Qiagen Genomic-tip 100/G columns and Blood & Cell Culture DNA Midi kits (Qiagen, USA), using the manufacturer’s recommended protocol, from overnight cultures grown at 37°C in trypticase soy broth (Becton, Dickinson, USA)
Typhimurium) is one of the most common serovars encountered in clinical settings, notably with the global expansion of a multidrug-resistant DT104 phagetype clone in the last few decades (2)
Typhimurium are carried on mobile genetic elements and plasmids, complete genome sequence data allow the construction of improved phylogenetic trees that may be used to characterize the distribution and evolution of these elements

Summary

Introduction

Genomic DNA was purified with Qiagen Genomic-tip 100/G columns and Blood & Cell Culture DNA Midi kits (Qiagen, USA), using the manufacturer’s recommended protocol, from overnight cultures grown at 37°C in trypticase soy broth (Becton, Dickinson, USA). Typhimurium) is one of the most common serovars encountered in clinical settings, notably with the global expansion of a multidrug-resistant DT104 phagetype clone in the last few decades (2). S. Typhimurium can be isolated from a broad range of sources, including produce, dairy, pork, poultry, and beef (3). Typhimurium are carried on mobile genetic elements and plasmids, complete genome sequence data allow the construction of improved phylogenetic trees that may be used to characterize the distribution and evolution of these elements.

Results

Conclusion