Complete Circular Genome Sequences of Three Bacillus cereus Group Strains Isolated from Positive Blood Cultures from Preterm and Immunocompromised Infants Hospitalized in France.

Mariem Ben Khedher,Fredrik Nindo,Florence Fenollar,Alicia Chevalier,Olivier Croce,Raymond Ruimy,Gregory Dubourg,Romain Lotte,Florence Casagrande,Stéphane Bonacorsi,Laurent Boyer,Jean-Marc Rolain,Armel Gallet

doi:10.1128/mra.00597-21

Abstract

ABSTRACTWe report here the complete genome sequences of three Bacillus cereus group strains isolated from blood cultures from premature and immunocompromised infants hospitalized in intensive care units in three French hospitals. These complete genome sequences were obtained from a combination of Illumina HiSeq X Ten short reads and Oxford Nanopore MinION long reads.

Highlights

Data for strain: Characteristic Bacterial speciesb MLST Isolation date Country: city BioSample accession no
AMLST, multilocus sequence type; ST, sequence type; ONT, Oxford Nanopore Technologies. bStrain identification by $96% similarity or $70% with digital DNA-DNA hybridization
The Illumina and Nanopore reads were used as the input for a hybrid de novo genome assembly and circularization of the replicons using the Unicycler v04.9 pipeline (8) with default parameters

Summary

Introduction

Data for strain: Characteristic Bacterial speciesb MLST Isolation date (yr/mo/day) Country: city BioSample accession no. Of reads (Illumina) Genome size (bp) Topology No of full genome sequences (chromosome 1 plasmids) N50 (bp) G1C content (%) Total no. AMLST, multilocus sequence type; ST, sequence type; ONT, Oxford Nanopore Technologies.

Results

Conclusion