Abstract
The green alga Botryococcus braunii is capable of the production and excretion of high quantities of long-chain hydrocarbons and exopolysaccharides. In this study, we present the complete plastid and mitochondrial genomes of the hydrocarbon-producing microalga Botryococcus braunii race B (Showa), with a total length of 156,498 and 129,356 bp, respectively.
Highlights
The green alga Botryococcus braunii is capable of the production and excretion of high quantities of long-chain hydrocarbons and exopolysaccharides
The mitochondrial genome of B. braunii Showa has a length of 129,356 bp and a GϩC content of 50.41%
The gene order and content is similar to the mitochondrial genome of another recently published Botryococcus braunii Showa strain (84.58 kb [12]), based on average nucleotide identity (ANI) analysis both mitochondrial genomes show only 75.9% similarity [10, 11]
Summary
The green alga Botryococcus braunii is capable of the production and excretion of high quantities of long-chain hydrocarbons and exopolysaccharides. For the establishment of the Botryococcus braunii race B (Showa) organelle genome sequences, purified DNA was used to construct a paired-end sequencing library (Illumina, USA). For the circularization an in silico gap closure approach [5,6,7] was applied, resulting in circular replicons for both plastid and mitochondrial genome sequences of Botryococcus braunii Showa.
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