Abstract
Chemical modification of all 18 car☐yl groups in Golfingia gouldii hemerythrin was achieved using glycine methyl ester and 1-ethyl-3-dimethyl-aminopropylcarbodiimide. The absorption spectrum of the modified protein in the visible and near ultraviolet region was the same as that of unmodified hemerythrin, indicating that car☐yl groups are not linked to iron in the active center. Modification of all 18 or as few as 8 car☐yl groups resulted in dissociation of the octameric hemerythrin into monomers. Analysis of mixtures of octamer and monomer produced by partial modification suggest the involvement of one specific car☐yl group in the observed dissociation reaction.
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