Abstract
Saffron, a spice derived from Crocus sativus, which in Iran is subjected to different trimming, is known for its beneficial health effects and high market value. Authentication studies related to geographical origin and adulterants presence mainly exist in literature, however fraud due to trimming has not been reported. In the current research, chemical characterization of six saffron trims, namely Sargol, Negin, Pushal, Bunch, Style, and Powder, was accomplished through suspect and non-target screening employing LC-QToF-MS in both electrospray ionization modes. The samples were extracted using methanol:water (50:50,v:v) and 62 compounds were identified, including amino acids, vitamins, flavonoids, phenolics, carotenoids, cyclohexenones. A clear discrimination among the red trims (Pushal, Sargol and Negin), as well as between Style and Bunch using Multivariate Chemometrics techniques was achieved. Proline and isophorone were highlighted as authenticity markers. Finally, the effect of three harvesting year on the most contributing compounds for trimming discrimination has been evaluated.
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