Abstract
The chicken hepatic lectin is involved in the clearance of glycoproteins from circulation (Kawasaki, T., and Ashwell, G. (1977) J. Biol. Chem. 252, 6536-6543). The complete amino acid sequence of chicken hepatic lectin has been established by analysis of peptides generated by chemical cleavage at methionine or tryptophan residues. Larger BrCN fragments were further digested with trypsin, chymotrypsin, and clostripain. All sequences were determined by automated sequential Edman degradation. Extensive use was made of high performance liquid chromatography in the purification of peptides and identification of phenylthiohydantoin derivatives of amino acids. The complete sequence is: (formula: see text). The stretch of uncharged amino acids from residue 25 to 48 is a possible membrane-interaction region. Carbohydrate is attached to residue 67.
Highlights
The chicken hepatic lectin is involved in the clearance of glycoproteins from circulation (Kawasaki, T., and Ashwell, G . (1977) J Biol
Rapid transfer of partially desialated rat serum glycoproteins from plasma to liver has beenextensively studied by Ashwell and Morrell (1)and colleagues
These investigators have isolated a carbohydrate-binding protein from mammalian liver (2). This “lectin” appears to mediate the specific binding phase of the clearance phenomenon(3). It recognizes the terminal galactose residueexsposed upon removalof sialic acid from glycoproteins
Summary
’ Portions of this paper (including “Experimental Procedures,”part of “Results,” Figs. 3 to 21, Tables I to XXXI, and additional references) are presented in miniprint at the end of this paper. 3 to 21, Tables I to XXXI, and additional references) are presented in miniprint at the end of this paper. Miniprint is read with the aid of a standard magnifying glass. Full size photocopies are available from the Journal of Biological Chemistry, 9650 Rockville Pike, Bethesda, MD 20014. Request Document No 80M-2044, cite author(s), and include a check or money order for $25.20 per set of photocopies. Full size photocopies are included in the microfilm edition of the Journal thaits available from Waverly Press.
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