Abstract
The complete 1H- and 13C-n.m.r. assignments for β- d-Gal p-(1→4)-β- d-Glc pNAc-6-SO 3H-(1→6)-[β- d-Gal p- (1→3)]- d-GalNAcol and α-NeuAc p-(2→3)-β- d-Gal p-(1→3)-[β- d-Gal p-(1→4)-β- d-Glc pNAc-6-SO 3H-(1→6)]- d-GalNAcol were made by a combination of 2-D correlation experiments (Relayed-Cosy; and 13C, 1H Correlation-shift n.m.r. spectroscopy), and 1-D n.m.r. spectroscopy. The results illustrate the ability of these methods to locate sulphate and NeuAc groups in anionic mucinous glycoproteins.
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