Abstract
Production of type 4 adeno-associated satellite virus (ASV) structural antigens and infectious DNA was studied using temperature-sensitive ( ts) mutants of herpes simplex virus (HSV) types 1 and 2. Seven of the eight mutants studied were unable to synthesize HSV DNA at the nonpermissive temperature. Mutants belonging to three HSV-1 and two HSV-2 complementation groups complemented ASV antigen production while members of two other HSV-1 groups did not. Infectious satellite virus DNA was isolated from the HSV-1 ts-1 complementing system.
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